Universidad Rey Juan Carlos

Máster de formación permanente en reproducción humana

Block I – Female Sterility

• Epidemiology of reproduction
• Anatomy and physiology of ovarian function
• Study of the sterile couple
• Age of the woman and fertility
• Ovarian stimulation protocols in Human Reproduction
• Drugs in ovarian stimulation: clomiphene, gonadotropins , GnRH analogues, hCG.
• Low response to stimulation protocols
• High response to ovarian stimulation
• Ovarian hyperstimulation syndrome
• Endometriosis and reproduction
• Polycystic ovary syndrome: generalities, genes, metabolic connection and reproductive difficulties
• Ectopic pregnancy
• Hydrosalpinx and reproduction

Block II – Male Sterility

• Spermatogenesis: basic concepts, sperm evaluation
• Study of the sterile male
• Varicocele and infertility/Male sterility of genetic cause
• FISH and DNA Fragmentation: clinical utility
• HIV and reproduction
• Preservation of Social Fertility
• Preservation of Oncological Fertility
• Freezing and sperm bank

Block III – Gametes and Embryos

• Growth, maturation and fertilization of the oocyte
• Epigenetics in gametogenesis and early embryonic development
• Cocultivation of human embryos and Sequential Culture
• Embryo implantation
• The new In Vitro Fertilization laboratory: "cleanroom"
• Embryo quality as predictive value

Block IV – Assisted Reproduction Techniques

• Artificial insemination
• Current role of IVF in the era of micromanipulation
• Intracytoplasmic sperm injection (ICSI) as an assisted reproduction technique
• Genetic compatibility test: New frontiers in reproduction
• Clinical approach to failure of implantation
• Genetic counseling in ART
• Oocyte donation/ In Vitro Fertilization in uterine surrogacy
• Preimplantation Genetic Screening
• Preimplantation Genetic Diagnosis
• The immune system and implantation today

Block V – New Techniques

• Assisted hatching and related techniques / Transf. Nuclear and Cytoplasmic
• New techniques in the IVF laboratory
• Freezing of oocytes
• The “inadequate” endometrium: medical, surgical and experimental treatments
• Legislation and ethics in human reproduction

Block VI – Research in Assisted Reproduction

Scientific information and documentation

Design of research protocols. Methodological approach

Study of proteins: Immunohistochemistry, ELISA, flow cytometry and Western blot

Study of genes: Northern blot, Southern blot, RT-PCR, F-PCR, differential display, DNA microarrays